Journal: PLoS ONE

Article Title: Keratinocyte Growth Factor Induces Gene Expression Signature Associated with Suppression of Malignant Phenotype of Cutaneous Squamous Carcinoma Cells

doi: 10.1371/journal.pone.0033041

Figure Lengend Snippet: ( A ) Growth supplement-starved normal primary keratinocytes (Kerat63), serum-starved HaCaT cells and primary (UT-SCC-12A) and metastatic (UT-SCC-7) cutaneous SCC cells were treated with recombinant KGF (rKGF; 10 ng/ml) for 2 h followed by addition of BrdU. DNA-synthesis was determined as incorporation of BrdU into DNA after 18 h incubation. * p <0.02; ** p <0.005, with Mann-Whitney U-test, n = 5–6. ( B ) Keratinocytes (Kerat63) (n = 8), HaCaT cells (n = 12), UT-SCC-12A (n = 12) and UT-SCC-7 (n = 12) cells were treated as in (A). The relative amount of viable cells was determined using colorimetric assay of WST-1 reagent metabolism. * p <0.02; ** p <0.000001, with Mann-Whitney U-test. ( C ) Serum-starved primary human skin fibroblasts were infected with recombinant adenovirus (RAdKGF) coding for human KGF, or with empty control adenovirus (RAdpCA3) (MOI 200) overnight and incubated in DMEM containing 0.5% FCS for 72 h. The conditioned medium (c.m.) was analyzed for the presence of KGF by western blotting, and KGF concentration was quantified with ELISA. ProMMP-1 was visualized as control in c.m. by western blotting. ( D–F ) Serum-starved HaCaT cells ( D ), UT-SCC-12A cells ( E ) and UT-SCC-7 ( F ) cells were incubated with c.m. in indicated concentrations or with rKGF for 18 h. BrdU was added and DNA-synthesis was analyzed after 8 h incubation. * a p <0.05, ** a p <0.01, * p <0.05 compared to 0% c.m., ** p <0.02 compared to 0% c.m. with Mann-Whitney U-test, n = 4–5.

Article Snippet: KGF concentration in the media were determined by ELISA, Quantikine Immunoassay, human KGF (DKG00, R&D Systems, Minneapolis, MN, USA).

Techniques: Recombinant, DNA Synthesis, Incubation, MANN-WHITNEY, Colorimetric Assay, Infection, Control, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia

doi: 10.1016/j.celrep.2024.115056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse Keratinocyte Growth Factor, KGF/FGF-7 ELISA Kit , MyBioSource.com , Cat#MBS701004.

Techniques: Purification, Blocking Assay, Plasmid Preparation, Functional Assay, Control, Virus, Recombinant, Avidin-Biotin Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Effect of various treatments on hWJMSCs-Sec toward growth factor proteins level. A) KGF, B) HGF, C) HB-EGF, D) PDGF, E) EGF. The data were presented as mean ± standard deviation. Different superscript letters on Figure A (a, b, c, cd, d, e, f), and B, C, D, E (a, b, c, d, e, f, g) showed significant differences among treatments at p<0.05 (Data was analyzed using ANOVA followed by Tukey HSD post hoc test). Samples included hWJMSCs-Sec (S), Freeze-dried hWJMSCs-Sec (FDS), Freeze-dried Medium Basal (FDMB), Freeze-Dried hWJMSCs-Sec Gel (FDSG), and Freeze-dried Gel Based (FDGB). Number on samples indicate the ratio of gel to hWJMSCs-Sec, 1) 6 g : 3 ml , 2) 6 g : 4.5 ml , and 3) 6 g : 6 ml .

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: Standard Deviation

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Proposed mechanism of hWJMSCs-Sec gel as a wound healing agent. * The treatment of hWJMSCs-Sec gel into the wounded skin can regulate some growth factors such as KGF, HGF, HB-EGF, PDGF, and EGF. The upregulation of these growth factors can induce the production of collagen, which plays a crucial role to regenerate tissue formation. The high antioxidant hWJMSCs-Sec gel also has the ability to scavenge free radicals, and inhibit the excessive inflammation that can cause irritation, redness, and pain in the wound.

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: